PINCH 1 controls Akt 1 for regulating radiation sensitivity by inhibiting PP 1 α
نویسندگان
چکیده
Cell culture, radiation exposure and 2D and 3D colony formation assay. Cells were cultured in Dulbecco's Modified Eagle Medium (PAA; plus glutamax-I) supplemented with 10% fetal calf serum (Biochrom) and 1% non-essential amino acids (PAA) (1). Single doses of 200 kV X-rays (Yxlon Y.TU 320; Yxlon; 0.5 mm copper filter; ~1.3 Gy/min, 20 mA) were applied and measured using a Duplex dosimeter (PTW) (1). To evaluate two(2D)or three(3D)-dimensional clonogenic cell survival, we plated cells on or in laminin-rich extracellular matrix (lrECM (MatrigelTM); BD) as published (1).
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Significance of Thymosin β4 and Implication of PINCH-1-ILK-α-Parvin (PIP) Complex in Human Dilated Cardiomyopathy
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